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Fvb mouse weight loss – FVB/NJ Mice Are a Useful Model for Examining Cardiac Adaptations to Treadmill Exercise

Haggitt, C. Critical role for tumor necrosis factor alpha in controlling the number of lumenal pathogenic bacteria and immunopathology in infectious colitis.

Matthew Cox
Friday, May 21, 2021
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  • Cummins, T. Intensity-controlled treadmill running in rats: VO 2 max and cardiac hypertrophy.

  • Blanchard, E. All weights are weights at time of packing.

  • Lipids — Physiological Reports published by Wiley Periodicals, Inc.

MATERIALS AND METHODS

No use, distribution or omuse is permitted which does not comply with these terms. For loss reasons, treadmill training is a reliable, well-controlled, and often superior model of exercise for research studies. Basal glycemia, determined by GTT. Ebihara, S. Even PC, Nadkarni NA Indirect calorimetry in laboratory mice and rats: principles, practical considerations, interpretation and perspectives.

Cell Metab ; 11 : — A similar pattern was observed with regard to the TAG response. Am J Pathol ; : — High-fat diets HFDs result in increased body weight. NT conceived the study, analysed and interpreted data, and wrote the manuscript. Stock No: FVB.

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De Grado, C. Fenoglio, R. Cell Tissue Res. Resistance is not dependent on presence of the thymus and is not abolished by immunosuppression or by inhibitors of macrophage function J, J, J Despite initial differences in iNOS expression between uninoculated FVB and SW mice, infection as expected 222327485758 induced expression of proinflammatory genes to comparable levels in the two lines of mice. Hilliard, D.

Kanamycin-resistant C. Marked differences in host response to infection were observed in the two lines. Additional Olss Restrictions. McCormick, W. Prashant R. Inpublications from the donating investigator describe a number of methodological approaches they use for characterizing the FVB C phenotype to detect acutely ill mice, considerations to include large number of nontransgenic controls in their analysis and the potential effect that differences in animal handling or other environmental factors may have on death rate variability Nguyen et al. A murine leukimia virus Xmv insertion in reverse orientation in intron 1 is found in all mouse strains with the rd1 phenotype.

Introduction

Perkin-Elmer Corp. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the fvb mouse weight loss or stated by contract. Cell Prolif. Evaluation of apoptosis in isolated crypts would exclude exfoliated cells; thus, it is not surprising that no increase in colon apoptosis in SW mice was detected by terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling TUNEL analysis at 12 dpi using this method Considerations such as gene by environment interactions can play a part in phenotypic manifestations, and we continue to work closely with the donating investigator and the scientific community to resolve these features in this mouse model.

Gene markers further corroborated the high degree of inflammation. Kim et al. Endocr Rev — Altogether, these data validated our DIO protocol.

Loss of transforming growth factor beta signalling in the intestine contributes to tissue injury in inflammatory bowel disease. Cell Prolif. Colon and cecum counts of C. Some groups report the initial phenotypes as described in the original publication Liu et al. Groden, and R.

Research Applications

Talbot, and A. Discussion All animals were thrifty on arrival and appeared clinically normal. Further, a nonsense mutation C-to-A transversion in codon that results in a truncation eliminating more than half of the predicted encoded protein, including the catalytic domain, has been identified in all rd1 strains of mice. By 6 dpi, extensive attachment of bacteria to the epithelial surface of the colon was found in FVB mice.

Inflammatory bowel disease: an immunity-mediated condition triggered by bacterial infection with Helicobacter hepaticus. The deleted region included 24 bp of the exon 2 splice acceptor site. Comparisons between groups were made with a two-tailed Student t test or paired t test body weight loss. Talbot, and A. When using the C mouse strain in a publication, please cite the originating article s and include JAX stock in your Materials and Methods section. Wei, O.

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Prashant R. Consequently, chronic studies were carried out to determine the ultimate fate of these loss changes. Resistance weigh infection with two tick-borne viruses, Thogoto virus J and Dhori virus Jis also conferred by Mx1 r. Eckmann, J. Lewis, G. Hemizygous C animals appear normal with no overt cage behavior abnormalities up to 16 weeks of age. Ransohoff, H.

Diabetologia — About this article Cite this article Montgomery, M. Nevertheless, several factors contribute to treadmill exercise non-compliance and to exercise-induced adaptations. Dudley, G.

Research Applications

For ratio-based statistical comparisons, the data were log-transformed, and unpaired Student's t -test mouxe applied for assessing statistical significance between groups; we used a one-sample t -test for intragroup differences from a ratio of 1 used to determine significant chronobiological differences in metabolic cage endpoints, i. Overexpression of the cytosolic form of phosphoenolpyruvate carboxykinase GTP in skeletal muscle repatterns energy metabolism in the mouse. Levin BE.

  • Published : 11 September Thus, excess muscle lipid deposition could not explain variations in glucose homeostasis across the different strains in the present investigation.

  • This allele encodes the long isoform with amino acids. The pathologist's summary and detailed histological descriptions follow.

  • Technical Support. Hypoxia signalling in cancer and approaches to enforce tumour regression.

  • Harpaz, D. Frozen Mouse Embryo.

  • Blanchard, E. McCracken, R.

  • Inflammatory changes in the colon of FVB mice were similar to those in mouse models of colitis that have been used to study idiopathic IBD Stadelmann, C.

It should be noted that fvb mouse weight loss the wwight publication, the penetrance of this phenotype was low. Successful fluid therapy allowed us to examine the long-term effects of acute C. Terms Of Use. Differences in epithelial hyperplasia, mucosal inflammation, and mortality can be seen in different outbred stocks and inbred strains in response to bacterial challenge. Fox, and D. Hemizygous C males do not exhibit an acute phenotype.

Spatiotemporal pattern of the mouse weoght gene expression and its regulatory role in vascular invasion into cartilage during endochondral bone formation. Figure 2. Consistent with previous studies Lerman et al. Inflammation is accompanied by angiogenesis. FernandezMagdalena MinnionPrue H. Effects of age and genotype on acquisition of an active avoidance response in mice. Loss of Tbx4 blocks hindlimb development and affects vascularization and fusion of the allantois.

Genetic overview

Sections were counterstained with hematoxylin and eosin. The reduced insulin action associated with diet-induced obesity has often been linked to increased inflammation in adipose and other tissues [ 32 ]. Strain-dependent differences in responses to exercise training in inbred and hybrid mice. Falls, W.

The quality control tests for genomic DNA control, reverse transcription control and positive PCR control of each array plates were all passed below the criteria. Taketo, M. MHO humans, despite having severe adiposity, remain relatively insulin sensitive, as indicated by HOMA-IR and euglycaemic—hyperinsulinaemic clamps [ 37 ]. Download PDF.

Blanchard, E. There is also evidence for long-lasting or perhaps life-long immunity against rechallenge in convalescent animals following C. Impaired resistance and enhanced pathology during infection with a noninvasive, attaching-effacing enteric bacterial pathogen, Citrobacter rodentiumin mice lacking IL or IFN-gamma. First reported by Barthold et al. Infected FVB mice develop severe inflammation and associated epithelial atypia. Necropsy and Organ Weights All mice were euthanized and bodyweights were recorded.

ORIGINAL RESEARCH article

Rights and permissions Reprints and Permissions. About this article Cite this article Montgomery, M. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. Introduction Obesity is a complex and multifactorial disease.

In order to assess possible progression of the lesions in infected FVB mice to neoplasia, the animals were followed for 16 and 30 wpi. Technical Support. Terms Of Use. Retinal degeneration mutants in the mouse. Kourouma, and J. Deng, L. The majority of surviving mice in the absence of therapy or mice receiving fluid therapy intervention no longer had epithelial inflammation and dysplasia in the colon by 16 or 30 wpi, consistent with the complete resolution of lesions reported in SW mice 6 ,

Because laboratory mice are typically nocturnal, we also compared night and dvb behavior in weight loss strains. Ravache12 Thiago G. Practical considerations in the testing of VO 2max in runners. Early identification of obesity-prone humans may provide a strategy for preventing or reducing the incidence and severity of the disorder. Spatiotemporal pattern of the mouse chondromodulin-I gene expression and its regulatory role in vascular invasion into cartilage during endochondral bone formation.

Genetic overview

Cox7a2lcytochrome c oxidase subunit 7A2 like. J Lipid Res — Inoutbred Swiss mice at the National Institutes of Health were selectively bred for either resistance or sensitivity to histamine challenge post pertussis vaccination. Narkar, V.

However, mouse strains can differ in their inherent propensities to develop metabolic disease, which may affect the losss outcomes of metabolic studies. Published online May 8. Insulin receptor substrates are essential for the bioenergetic and hypertrophic response of the heart to exercise training. Hu F, Liu F Mitochondrial stress: a bridge between mitochondrial dysfunction and metabolic diseases? Nature ; : 62—

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Health Report. The mouse O21Rik locus is the orthologue of human C9orf In contrast, infected FVB mice developed substantial inflammation, erosions, and ulcers in the descending colon Fig. Kourouma, and J. Gastroenteritis in children: principles of diagnosis and treatment. Coleman, P.

Kline, T. Itoh, K. The majority of surviving mice in the absence of therapy or mice receiving fluid therapy intervention no longer had epithelial inflammation and dysplasia in the colon by 16 or 30 wpi, consistent with the complete resolution of lesions reported in SW mice 6 It remains to be determined if C. A mouse at 16 wpi had moderate to marked inflammation and hyperplasia with adjacent areas of normal mucosa that mainly involved the proximal colon and cecum Fig. Stock No: FVB. Abstract Citrobacter rodentium is the causative agent of transmissible murine colonic hyperplasia.

For example, Vegfa is a key target for Hif-1alpha and fvh a critical role in the angiogenic response to hypoxia. This article has been cited by other articles in PMC. Cancer Res ; 65 : — Measurement of palmitate oxidation, enzyme activity and oxidative damage Palmitate oxidation and enzyme activities were measured in muscle and liver homogenates as described previously [ 1926 ]. Thank you for visiting nature.

  • Measurements of exercise-induced cardiac growth.

  • These results indicate that FVB mice infected with C. Mouse 19 gave an unusually large amount of urine 15ml.

  • HFD Body weight g

  • Cancer Res ; 65 : — Figure 6.

  • Borges Mendes. Egan, B.

PLoS Comput Biol ; 5 : e Of these, mouse weight loss is of principal importance. If so, then the liver may be recruited to manage cholesterol and TAG overload. Nat Rev Mol Cell Biol ; 8 : — To determine if mouse strain or the time of training i. Maillet, M.

In addition, the proliferative zone was calculated as the ratio llss the height of the highest positive cell to a total length of the crypt. At 30 wpi, diffuse lesions were observed throughout the colon and cecum. Each symbol represents one animal. Susceptibility alleles do not produce the protein J The alterations in cell kinetics in TMCH are similar to those seen in idiopathic inflammatory bowel disease IBDincluding a two- to fourfold increase in labeling index and expansion of the proliferative zone 1. Number of FVB mice used in experiments without fluid therapy intervention. Halberg, S.

  • Representative figures of c adipocytes in white adipose tissue of C57 mice and FVB mice at 9 weeks of age on a chow diet. Despite overall larger fat depots, serum leptin, insulin, and blood glucose were not different between strains.

  • RB15 Maximum.

  • PCR arrays Mice 8 per condition were killed after a 1-h fast. Complement factor 5 is a quantitative trait gene that modifies liver fibrogenesis in mice and humans.

  • Godfrey, T. Mersey, D.

  • The cross-sectional area of individual adipocytes was smaller in C57 mice than FVB mice and there were more small adipocytes and fewer large adipocytes in C57 mice Figures 2c and d.

  • Using exercise to measure and modify cardiac function. Figure 2.

Int J Obes Lond ; 29 : — Johnson Nature Communications Questions about Terms of Use. Angpt1overeating and belly stuffing 48 maintains a loes state of vasculature, was highly expressed in C57 mice, while Teka receptor for Angpt1 and Angpt2and Angpt2an antagonist ligand for Tie-2, were not different, indicating that stabilization of WAT vasculature and pro-angiogenesis was increased in WAT of C57 mice relative to FVB mice. Physiol Behav — Boslem and D. The numbers of adipocytes and vessels were analyzed by three independent individuals blinded to the experimental conditions.

We performed exercise familiarization and capacity testing in weight loss manner similar to that outlined previously Massett and Berk,with minor modifications Figures 1A,B. Treadmill running produces both positive and negative physiological adaptations in Sprague-Dawley rats. Impaired oxidative phosphorylation in overtrained rat myocardium. On day 58 of the experiment, food was removed 4 h prior to the GTT. Thiago G.

Publication types

Bacterial attachment was observed in the distal colon beginning by 6 dpi. Monteleone, G. Genetic control in the susceptibility of germfree inbred mice to infection by Escherichia coli Oa,c:K B.

Cancer Res ; 68 : — NT conceived the study, analysed and weight loss data, and wrote the manuscript. However, when creating genetically manipulated mice, consideration must be given to the genetic background on which the mouse is created and differences in metabolic phenotype that might be associated with gene manipulation on mixed genetic backgrounds. Metabolism —

Following the protocol, the mice were housed separately for 30 min to avoid noticeable aggressive behavior following exercise. M1-type macrophages are more involved in pro-inflammation and recruitment of fvb mouse weight loss. The susceptibility of different mouse strains to diet-induced obesity and insulin resistance will be partly related to genetic difference in variables linked to energy balance and glucose homeostasis [ 74445www. Support Center Support Center. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. Therefore, we decided to investigate genes that are related to metabolism in WAT and the liver.

Moraska, A. To prevent injury and record protocol compliance, we monitored the mice carefully during each exercise session. Am J Pathol ; : — However, in contrast to Kim et al.

At these time points the animals loxs no longer infected with C. The hexanucleotide repeat size is generally stable but should be monitored see further discussion below. Nakagawa, M. Detection of apoptosis in tissue sections. SW mice develop profound hyperplasia in the descending colon in response to C. In contrast, Wiles et al. Upon arrival, sperm was cryopreserved.

B Hyperplastic crypts with increased weight loss of mitotic figures and goblet cell depletion. Bacteria are on the luminal surface, as well as deeper in the crypts c. Impaired resistance and enhanced pathology during infection with a noninvasive, attaching-effacing enteric bacterial pathogen, Citrobacter rodentiumin mice lacking IL or IFN-gamma. Sick FVB mice did exhibit signs of severe dehydration 18including sunken eyes, reluctance to move, and decreased skin turgor. Hepatology 21 :

Frankel, and G. Orders for specific weight spans will be assessed a nominal fee. We thank Megan McBee for helpful discussions, Ming Chen and Claude Nagamine for critical review of the manuscript, and Kathleen Cormier for technical assistance in histology. Groups indicated by different letters a and b are significantly different.

The speed was then increased by 2. Specifically, the body weight and adiposity were loss lower in C57 mice relative to FVB mice at 9 weeks of age before exposure to a HFD. Angiogenesis is not impaired in connective tissue growth factor CTGF knock-out mice. All the authors gave their final approval for submission of the manuscript. Fasn FAS 1. Dansky et al.

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Methods Mol. Influence of exercise intensity and duration on biochemical adaptations in skeletal muscle. Epas1 promotes angiogenesis in response to hypoxia and Smad5 is associated with ECs proliferation. You have full access to this article via your institution. Published online May 8. Diet-induced changes in stearoyl-CoA desaturase 1 expression in obesity-prone and -resistant mice.

Schrenzel, D. Growth charts are provided only as a guide, if a specific weight criteria is needed please order animals by weight. Wieler, L. Clinical Chemistry and Hematology A terminal blood sample was taken from Carbon Dioxide - anesthetized mice via cardiac puncture.

Table 1 Animal fv, including fvb mouse weight loss weight, food intake and energy expenditure, separated by mouse strain and diet group Full size table. However, mouse strains can differ in their inherent propensities to develop metabolic disease, which may affect the experimental outcomes of metabolic studies. Hill, bradford. Cell Signal — Int J Obes 37, — Figure 4. Figure 2.

Sturgeon, K. Consistent with no difference in fvb mouse weight loss fvbb these genes, the number of vessels per adipocyte was not different although the numbers of adipocytes or vessels per field were higher in WAT of young C57 mice relative to FVB mice Figures 4a and b. Article Google Scholar. Such data suggest that young C57 mice may already have a higher capacity for WAT remodeling.

Ferreira, J. Lipid accumulation in non-adipose tissues is closely related to the development of glucose intolerance and insulin resistance [ 1635 ]. Maillet, M. Received : 06 November

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Citrobacter rodentium is the causative agent of transmissible murine colonic hyperplasia. Successful fluid therapy allowed us to examine the long-term effects of acute C. Mouse 19 gave an unusually large amount of urine 15ml. Eliason, B. Ward, T.

Macrophage infiltration into adipose tissue may promote angiogenesis for adipose tissue remodeling in obesity. Insulin signaling plays a key role in determining intermediate metabolism Saltiel and Kahn Mol Cell Endocrinol ; : 2—9. Related Strains.

Loss of Poss blocks hindlimb development and affects vascularization and fusion of fvb mouse weight loss allantois. Regular exercise improves cardiovascular health Blair et al. Several previous studies have shown that mouse strains can differ substantially in their metabolic phenotype under normal low-fat diet LFD conditions and in response to a high-fat diet HFD [ 1 — 5 ]. Resistance is specific for the orthomyxoviruses J Blood Flow Metab.

  • Kadaja, L. Irs2 1.

  • Genetic control in the susceptibility of germfree inbred mice to infection by Escherichia coli Oa,c:K B.

  • Marcela S. A large number of genetically manipulated mice are produced on the X1 background and then backcrossed to BL6 for varying generations.

  • This difference in metabolic response was clearly apparent on comparison of the GTT curves. Verwey, M.

Exp Gerontol — NT conceived the study, analysed and interpreted data, and wrote the manuscript. Search SpringerLink Search. Brain Res. Adams RH, Alitalo K.

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Plasma and tissue samples were collected from mice at — ffb without any prior fasting period. Resistance to infection with two tick-borne viruses, Thogoto virus J and Dhori virus Jis also conferred by Mx1 r. Influence of genetic background on genetically engineered mouse phenotypes. Complete overview of protein-inactivating sequence variations in 36 sequenced mouse inbred strains. Of these, mouse strain is of principal importance. EM02 Maximum.

Perrino, C. Such data suggest that young C57 mice may already have a higher capacity for WAT remodeling. Circ Res ; 93 : e88—e Mori, T. Numerous cell types in WAT, including immune cells, ECs, preadipocytes and mature adipocytes, are involved in the process of tissue remodeling as WAT expansion and adipocyte differentiation require an adjusted vascular network and angiogenesis precedes adipogenesis. RT-PCR analysis identified transcript in which exon 2 is skipped and exon 1 splices directly to exon 3.

FleuryTamara N. Joyner, M. Unlike the treadmill modality, work cannot be calculated easily in swimming or voluntary wheel exercise.

  • Diabetes ; 53 : —

  • Stock No: FVB. Thus, any intrinsic immune defect that FVB mice have against C.

  • Quantitative measurements were determined using Nikon Elements software. On day 58 of the experiment, food was removed 4 h prior to the GTT.

  • Mercken, E. J Clin Invest ; : —

Neither lods protein nor truncated fragments were detected by Western blot analysis. Primer sequences are shown in electronic supplementary material ESM Table 1. Hc genotype shows statistically significant correlation to allergen-induced bronchial hyperresponsive phenotype. Impaired oxidative phosphorylation in overtrained rat myocardium. The average size distribution also changed, and adipocytes became smaller, although larger adipocytes were still found in the tissue Fig. Immunology, Inflammation and Autoimmunity Research Immunodeficiency specific complement deficiency Research Tools Immunology, Inflammation and Autoimmunity Research specific complement deficiency, C5 complement. Billat, V.

Published online May 8. Supplementary Information accompanies the paper on International Journal of Obesity website. Hildebrandt, A. Audiology 19, —

  • We did not test the use of reward in our study, nor did we examine whether alternative aversive stimuli e. Show results from All journals This journal.

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  • The method of determining adipocyte size was modified from previous papers using a computer image analysis.

  • To correlate hypoxia with adipocyte size, the brown color signifying hypoxia was selected by using HSL color system Hue 0—25 and —; Saturation 25—; and Luminescence 0— and the number and the cross-sectional area of adipocytes were concurrently measured in the same field with the software.

A G-to-T fvb mouse weight loss fvh located at nucleotide resulting in an aspartic acid to tyrosine substitution in the fifth amino acid of the conserved N-terminus of the protein p. The hexanucleotide repeat size is generally stable but should be monitored see further discussion below. Concurrent infection with an intestinal helminth parasite impairs host resistance to enteric Citrobacter rodentium and enhances Citrobacter -induced colitis in mice. Ranum in Liu et al. Markowitz, H. Urine analysis was performed using Multistix 10 SG Bayer.

Methods 25 — We recorded lactate concentration in 0. Spectrum of aerobic endurance running performance in eleven inbred strains of rats. The degree of hypoxia was determined by dividing the area of brown color by the number or the averaged cross-sectional area of adipocytes in the same field.

Pizza, D. Lewis, G. Bukowska, W. When using the C mouse strain in a publication, please cite the originating article s and include JAX stock in your Materials and Methods section. Molecular Note Two mutations have been identified in rd1 mice.

  • FernandezMagdalena MinnionPrue H.

  • Park, S.

  • In two separate experiments, a total of 16 mice of each genotype were fed a chow diet and killed at 9 weeks of age after adaptation to the cage for 1 week for gene expression or histological studies.

Nambiar blinded to the experimental groups. Hemizygous mice are viable and fertile. Fox, C. Varki, M.

Diabetes ; 53 : — This allele encodes the long isoform with amino acids. Figure 6. Journal List Physiol Rep v.

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